An empirical assignment of T1 Raman lines
نویسندگان
چکیده
*sN-substituted bacteriochlorophyll a (BChl a) was extracted from the cells of Rhodobacter sphaeroides 2.4.1 grown in a medium containing 15N-ammonium sulfate and yeast concentrate. The T, Raman spectra of 14Nand “N-BChl a were obtained as the difference spectra of high-power minus low-power of one-color, pump-and-probe measurements using 420 nm, 5 ns pulses. A set of empirical assignments of the TI Raman lines was made, based on shifts upon 14N+lSN substitution. The S,, Raman spectra of the two BChls were also obtained by using the 457.9 nm cw beam, and a set of assignments of the So Raman lines was given for comparison.
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